Cerebellar foliation via non-uniform cell accumulation caused by fiber-guided migration of granular cells

The cerebellum has a unique morphology characterized by fine folds called folia. During cerebellar morphogenesis, folia formation (foliation) proceeds with granule cell (GC) proliferation in an external granular layer, and subsequent migration to internal layer (IGL). GC is guided along Bergmann glial (BG) fibers, whose orientation depends on the deformation of tissue during formation. aim this study investigate contribution fiber-guided from mechanical viewpoint. Based continuum mechanics model dynamics, we simulated foliation process caused migration. By changing speeds, showed that non-uniform accumulation GCs lengthening. Furthermore, simulation impaired under pathological conditions, where did not migrate BG revealed was necessary for These results successfully recapitulated features physiological processes validated mechanisms guidance fibers causes lengthening accompanied IGL. Our computational approach will help us understand biological physical morphogenesis mechanisms, facilitated interactions between cellular activities behaviors.